mbd2b protein  (New England Biolabs)

Journal: Epigenomics

Article Title: MIRA-seq for DNA methylation analysis of CpG islands

doi: 10.2217/epi.15.33

Figure Lengend Snippet: (A) MIRA-seq was performed on DNA from normal melanocytes (N, green) and three melanoma samples (T1, T2, T3, blue). The vertical arrow indicates the region of the HOXA9 gene analyzed by bisulfite sequencing. (B) Bisulfite sequence data for normal melanocytes. (C–E) Bisulfite sequence data for three melanoma tumors. Open circles: unmethylated CpG sequences; black circles: methylated CpG sequences. The percentages indicate percentage of methylated CpGs.

Article Snippet: A kit containing recombinant MBD2b and MBD3L1 proteins for enrichment of methylated DNA is currently available as 'MethylCollectorTM Ultra' from Active Motif (CA, USA).

Techniques: Methylation Sequencing, Sequencing, Methylation

Journal: ILAR Journal

Article Title: Developmental Epigenetics of the Murine Secondary Palate

doi: 10.1093/ilar.53.3-4.240

Figure Lengend Snippet: A schematic outline to identify differentially methylated genes using promoter microarrays. Genomic DNAs from secondary palates (gestation days 12–14) were isolated and fragmented by sonication. Sonicated DNA was incubated with MBD2b, a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments. The enriched DNA fragments from all three gestational days were amplified by whole genome amplification. Genomic DNAs not subjected to methylation enrichment were used as controls. Control DNA, which was labeled with Cy3, and experimental DNA, which was labeled with Cy5, were hybridized to NimbleGen 2.1 M mouse promoter microarrays (Roche NimbleGen, Madison, WI). Nine microarrays representing three biologic replicates on each of gestation days 12, 13, and 14 were probed. After hybridization and washing, the microarray chips were scanned and the accrued data were analyzed using biostatistical methods (Seelan et al., unpublished data).

Article Snippet: Sonicated DNA was incubated with MBD2b, a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments.

Techniques: Methylation, Isolation, Sonication, Incubation, Binding Assay, Amplification, Whole Genome Amplification, Control, Labeling, Hybridization, Microarray